Jing Lü Haicui Suo Rong Yi, Qibin Ma and Hai Nian
BMC Plant Biology 2015, 15:232 doi:10.1186/s12870-015-0602-6; 29 September 2015
ABSTRACT
Background
The precise timing of flowering is fundamental to successful reproduction, and has dramatic significance for crop yields. Although prolonged low temperatures are not required for flowering induction in soybean, vernalization pathway genes have been retained during the evolution of this species. Little information is currently available in regarding these genes in soybean.
Results
We were able to detect the expression of Glyma11g13220 in different organs at all monitored developmental stages in soybean. Glyma11g13220 expression was higher in leaves and pods than in shoot apexes and stems. In addition, Glyma11g13220 was responsive to photoperiod and low temperature in soybean. Furthermore, Glyma11g13220 was found to be a nuclear-localized protein. Over-expression of Glyma11g13220 in an Arabidopsis Columbia-0 (Col-0) background resulted in early flowering. Quantitative real-time PCR analysis revealed that transcript levels of flower repressor FLOWERING LOCUS C (FLC), and FD decreased significantly in transgenic Arabidopsis compared with wild-type Col-0, while the expression of VERNALIZATION INSENSITIVE 3 (VIN3) and FLOWERING LOCUS T (FT) noticeably increased.
See: http://www.biomedcentral.com/1471-2229/15/232
![Glyma11g13220, a homolog of the vernalization pathway gene VERNALIZATION 1 from soybean [Glycine max (L.) Merr.], promotes flowering in Arabidopsis thaliana](/Images_upload/images/New Picture (4)(40).png)
Fig. 4. Expression patterns of Glyma11g13220 under different light conditions. All seedlings were grown under short-day conditions until 10 days after emergence (DAE), at which point half of the seedlings were transferred to long-day conditions. Fully expanded trifoliate leaves were sampled from three individual plants growing under short- and long-day conditions 12 h after dawn at 12, 15, 18, 21, 24, 27 and 30 DAE. a Image obtained approximately 53 DAE (SD, short-day conditions; LD, long-day conditions). b Quantitative real-time PCR analysis of Glyma11g13220 under short- and long-day conditions at 12, 15, 18, 21, 24, 27 and 30 DAE. Expression levels are normalized to Gmβ-tubulin (Glyma20g27280). Values are means ± SD of three biological replicates, with each measurement repeated three times. Significant differences based on the t-test are denoted by asterisks: * p < 0.05, ** p < 0.01
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